Journal: bioRxiv
Article Title: Differences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Clean-up Techniques
doi: 10.1101/2024.03.13.584881
Figure Lengend Snippet: Comparisons of the average number of identified peptides (A) and proteins (B) for MCF7 breast cancer whole-cell lysates and subcellular fractions using SP3, SP4, SP3(detergent-assisted (DA)), and SP4(DA). Samples are separated by dashed lines with labels at the top of the figure. Error bars represent the standard error of the mean. Bracketed lines above bar graphs show significance of pair-wise comparisons, represented by *p < 0.05, **p < 0.01, and ***p < 0.001. Comparisons without * notation are not significantly different and are within measurement uncertainty.
Article Snippet: Epithelial estrogen receptor positive (ER+) MCF7 breast cancer cells (ATCC HTB-22, Manassas, Virginia) were cultured in Dulbecco’s Modified Eagle Medium (Corning, Corning, NY) supplemented with 10 % fetal bovine serum (Gibco, Billings, MT) and 1 % penicillin/streptomycin (Gibco, Billings, MT).
Techniques: